The chamber is capable of aerosolising a wide range of human pathogens. For such studies we employ a 24 Jet Collison nebuliser (Acoem, UK) linked to a Welch WOB-L piston Vacum pump to ensure reliable microorganism aerosolization. Microorganisms are sampled using a MAS-100 NT® autosampler that can be positioned in multiple areas with variable air sample volumes at set time points. Samples are collected directly onto growth media so that accurate numbers of recovered microorganisms can be reported.